Ospreys prop, Ruth Lewis is the sole uncapped player called up to the national squad alongside 13 of her regional teammates.
Siwan Lillicrap has been handed the squad captaincy for the Six Nations campaign in recognition of the leadership skills she displayed in leading the side in their last four games. Cara Hope returns to the Welsh side after missing the Autumn series through injury. She's joined up front by hooker Kelsey Jones and the second row Osprey duo of Gwen Crabb and Natalia John. After an impressive run out last time, Ospreys in the Community trustee and flanker Robyn Lock returns to the fold.
Half backs Keira Bevan and Elinor Snowsill get the nod alongside Courtney Keight, Kerin Lake, Niamh Terry, Lauren Smyth and community hub officer, Kayleigh Powell to complete the Ospreys contingent.
Horsman, Lewis and Wyatt continue in their autumn coaching roles with Rowland Phillips continuing to take time out of the programme, while former England Sevens captain Ollie Phillips has been appointed to lead the Wales Women Sevens programme.