| Date | Time | Comp | Home |
| Away | Venue | TV/Att | Rept |
|---|
| August 2009 |
| Sat 22 Aug | 14:30 | F | Ospreys | 35 - 20 | Leeds Carnegie | Liberty Stadium | 6,025 | Report |
| Sat 29 Aug | 15:00 | F | Gloucester Rugby | 16 - 22 | Ospreys | Kingsholm Stadium | 7,145 | Report |
| September 2009 |
| Fri 4 Sep | 19:00 | ML | Connacht Rugby | 12 - 19 | Ospreys | The Sportsground | 1,982 | Report |
| Sat 12 Sep | 18:30 | ML | Ospreys | 16 - 20 | Ulster Rugby | The Liberty Stadium | 7,072 | Report |
| Fri 18 Sep | 19:05 | ML | Ospreys | 11 - 18 | Leinster | The Liberty Stadium | 7,182 | Report |
| Fri 25 Sep | 19:05 | ML | Glasgow Warriors | 16 - 26 | Ospreys | Firhill | 3,108 | Report |
| October 2009 |
| Sun 4 Oct | 16:00 | ML | Ospreys | 31 - 10 | Edinburgh | The Liberty Stadium | 7,033 | Report |
| Sun 11 Oct | 12:45 | HC-P3 | Leicester Tigers | 32 - 32 | Ospreys | Welford Road | 20,029 | Report |
| Sun 18 Oct | 12:45 | HC-P3 | Ospreys | 25 - 24 | ASM Clermont Auvergne | The Liberty Stadium | 9,238 | Report |
| Sat 24 Oct | 18:30 | ML | Cardiff Blues | 20 - 12 | Ospreys | Cardiff City Stadium | 16,341 | Report |
| Fri 30 Oct | 19:05 | ML | Ospreys | 9 - 9 | Glasgow Warriors | The Liberty Stadium | 6,661 | Report |
| November 2009 |
| Thu 5 Nov | 19:45 | LVC-P1,P4 | Ospreys | 17 - 19 | Northampton Saints | The Liberty Stadium | 4,322 | Report |
| Sat 14 Nov | 12:30 | LVC-P1,P4 | Bath Rugby | 21 - 11 | Ospreys | Recreation Ground | 10,789 | Report |
| December 2009 |
| Sat 5 Dec | 18:30 | ML | Ospreys | 19 - 14 | Munster | The Liberty Stadium | 8,077 | Report |
| Sat 12 Dec | 14:30 | HC-P3 | Viadana | 7 - 62 | Ospreys | Stadio Il Giglio | 8,348 | Report |
| Sat 19 Dec | 14:30 | HC-P3 | Ospreys | 45 - 19 | Viadana | The Liberty Stadium | 7,974 | Report |
| Sat 26 Dec | 14:05 | ML | Scarlets | 14 - 21 | Ospreys | Parc y Scarlets | 14,739 | Report |
| January 2010 |
| Fri 1 Jan | 17:15 | ML | Ospreys | 26 - 0 | Cardiff Blues | The Liberty Stadium | 14,476 | Report |
| Fri 8 Jan | 19:05 | ML | Ulster Rugby | P - P | Ospreys | Ravenhill | BBCw | More |
| Sat 16 Jan | 16:45 | HC-P3 | ASM Clermont Auvergne | 27 - 7 | Ospreys | Stade Marcel Michelin |
| Report |
| Sat 23 Jan | 13:35 | HC-P3 | Ospreys | 17 - 12 | Leicester Tigers | The Liberty Stadium | 15,626 | Report |
| Fri 29 Jan | 19:30 | LVC-P1,P4 | Newport Gwent Dragons | 40 - 19 | Ospreys | Rodney Parade | 6,027 | Report |
| February 2010 |
| Thu 4 Feb | 19:35 | LVC-P1,P4 | Ospreys | 21 - 17 | Leeds Carnegie | The Liberty Stadium | 2,557 | Preview |
| Sun 21 Feb | 17:00 | ML | Ospreys | 19 - 17 | Connacht Rugby | The Liberty Stadium | 5,053 | Report |
| March 2010 |
| Sun 7 Mar | 14:00 | ML | Edinburgh | 33 - 17 | Ospreys | Murrayfield | 2,329 | Report |
| Sat 27 Mar | 18:30 | ML | Newport Gwent Dragons | v | Ospreys | Rodney Parade | S4C | More |
| April 2010 |
| Fri 2 Apr | 19:05 | ML | Ospreys | v | Scarlets | The Liberty Stadium | BBCw, Setanta | More |
| Sat 10 Apr | 16:00 | HC-QF | Biarritz Olympique Pays Basque | v | Ospreys | Estadio Anoeta | Sky Sports | More |
| Fri 16 Apr | 19:05 | ML | Leinster | v | Ospreys | Royal Dublin Society | BBCw | More |
| 23/24/25 Apr |
| ML | Munster | v | Ospreys | Thomond Park |
| More |
| May 2010 |
| 1/2/3 May |
| HC-SF |
| v |
| TBA |
|
|
| 1/2/3 May |
| HC-SF |
| v |
| TBA |
|
|
| 7/8/9 May |
| ML | Ospreys | v | Newport Gwent Dragons | The Liberty Stadium |
| More |
| 14/15/16 May |
| ML-SF |
| v |
| TBA |
|
|
| 14/15/16 May |
| ML-SF |
| v |
| TBA |
|
|
| Sat 22 May | 18:00 | HC-F |
| v |
| Stade de France |
|
|
| 28/29/30 May |
| ML-F |
| v |
| TBA |
|
|
|
