| Date | Time | Comp | Home |
| Away | Venue | TV/Att | Rept |
| August 2012 |
| Fri 10 Aug | 20:00 | F | ASM Clermont Auvergne | 49 - 25 | Ospreys | Stade du Mas |
| Report |
| Sat 18 Aug | 15:00 | F | Ospreys | 10 - 21 | Bath Rugby | Liberty Stadium | 6,290 | Report |
| Fri 31 Aug | 20:05 | RDP12 | Benetton Treviso | 12 - 6 | Ospreys | Stadio Comunale di Monigo | 3,500 | Report |
| September 2012 |
| Sat 8 Sep | 18:30 | RDP12 | Ospreys | 13 - 16 | Ulster Rugby | The Liberty Stadium | 8,566 | Report |
| Fri 14 Sep | 19:05 | RDP12 | Ospreys | 10 - 28 | Glasgow Warriors | The Liberty Stadium | 7,159 | Report |
| Fri 21 Sep | 19:05 | RDP12 | Scarlets | 16 - 23 | Ospreys | Parc y Scarlets | 14,111 | Report |
| Sat 29 Sep | 18:30 | RDP12 | Ospreys | 30 - 15 | Munster Rugby | The Liberty Stadium |
| Report |
| October 2012 |
| Fri 5 Oct | 19:05 | RDP12 | Zebre | 16 - 34 | Ospreys | Stadio XXV Aprile | 2,500 | Report |
| Fri 12 Oct | 20:00 | HC-P2 | Ospreys | 38 - 17 | Benetton Treviso | The Liberty Stadium | 8,746 | Report |
| Sun 21 Oct | 15:00 | HC-P2 | Leicester Tigers | 39 - 22 | Ospreys | Welford Road | 20,224 | Report |
| Sat 27 Oct | 14:30 | RDP12 | Ospreys | 26 - 9 | Connacht Rugby | The Liberty Stadium | 8,231 | Report |
| November 2012 |
| Sun 4 Nov | 16:00 | RDP12 | Ospreys | 19 - 10 | Leinster Rugby | The Liberty Stadium | 9,246 | Report |
| Fri 9 Nov | 19:45 | LVC-P1,P4 | Ospreys | 33 - 27 | Gloucester Rugby | The Liberty Stadium | 5,738 | Report |
| Sat 17 Nov | 17:00 | LVC-P1,P4 | Exeter Chiefs | 23 - 13 | Ospreys | Sandy Park Stadium | 7,829 | Report |
| Fri 23 Nov | 19:05 | RDP12 | Edinburgh Rugby | 23 - 13 | Ospreys | Murrayfield | 3,128 | Report |
| Fri 30 Nov | 19:05 | RDP12 | Ospreys | 33 - 12 | Cardiff Blues | The Liberty Stadium | 8,169 | Report |
| December 2012 |
| Sat 8 Dec | 14:35 | HC-P2 | Toulouse | 30 - 14 | Ospreys | Stade Ernest Wallon | 17,865 | Report |
| Sat 15 Dec | 13:35 | HC-P2 | Ospreys | 17 - 6 | Toulouse | The Liberty Stadium | 10,110 | Report |
| Wed 26 Dec | 17:30 | RDP12 | Ospreys | 32 - 3 | Scarlets | The Liberty Stadium | 19,443 | Report |
| Mon 31 Dec | 16:15 | RDP12 | Newport Gwent Dragons | 3 - 14 | Ospreys | Rodney Parade | 7,018 | Report |
| January 2013 |
| Fri 4 Jan | 19:05 | RDP12 | Ospreys | 16 - 15 | Zebre | The Liberty Stadium | 7,891 | Report |
| Sun 13 Jan | 15:00 | HC-P2 | Ospreys | 15 - 15 | Leicester Tigers | The Liberty Stadium | 13,126 | Report |
| Sun 20 Jan | 16:00 | HC-P2 | Benetton Treviso | 17 - 14 | Ospreys | Stadio Comunale di Monigo |
| Report |
| Sat 26 Jan | 18:30 | LVC-P1,P4 | Newport Gwent Dragons | 18 - 14 | Ospreys | Rodney Parade | 4,607 | Report |
| February 2013 |
| Sun 3 Feb | 17:00 | LVC-P1,P4 | Ospreys | 12 - 16 | Harlequins | Brewery Field |
| Report |
| Fri 8 Feb | 19:05 | RDP12 | Ulster Rugby | 12 - 16 | Ospreys | Ravenhill | 10,900 | Report |
| Fri 15 Feb | 19:45 | RDP12 | Connacht Rugby | 22 - 10 | Ospreys | The Sportsground | 4,680 | Report |
| Fri 22 Feb | 20:00 | RDP12 | Ospreys | 24 - 7 | Edinburgh Rugby | The Liberty Stadium | 5,580 | Report |
| March 2013 |
| Sat 2 Mar | 18:30 | RDP12 | Munster Rugby | 13 - 13 | Ospreys | Thomond Park | 17,700 | Report |
| Fri 22 Mar | 19:05 | RDP12 | Ospreys | 52 - 19 | Newport Gwent Dragons | The Liberty Stadium | 9,935 | Report |
| Sat 30 Mar | 17:15 | RDP12 | Cardiff Blues | 16 - 23 | Ospreys | Millennium Stadium | 36,174 | Report |
| April 2013 |
| Sat 13 Apr | 18:30 | RDP12 | Ospreys | 28 - 3 | Benetton Treviso | The Liberty Stadium | 9,133 | Report |
| Fri 19 Apr | 19:35 | RDP12 | Glasgow Warriors | 35 - 17 | Ospreys | Scotstoun Stadium | 6,642 | Report |
| May 2013 |
| Fri 3 May | 19:45 | RDP12 | Leinster Rugby | 37 - 19 | Ospreys | Royal Dublin Society | 14,000 | Report |
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